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Fast-food chain Zensho Co. stopped serving “gyudon” — bowls of rice topped with seasoned beef — Thursday after it exhausted its beef inventory following the government’s ban on U.S. beef.

Zensho, a Tokyo-based firm that runs 480 Sukiya restaurants across Japan, is the second major gyudon chain to remove beef dishes from its menu, following Nakau Co., which stopped serving them Monday.

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