Shares of the Industrial Bank of Japan, Dai-Ichi Kangyo Bank and Fuji Bank were delisted from the first section of the Tokyo Stock Exchange on Friday.
The delisting comes ahead of the Sept. 29 creation of Mizuho Holdings Inc., a holding company, by the three banks.
Mizuho Holdings, which will be the world's largest financial group with assets of 40 trillion yen, will go public on the first section on Thursday.
It will be the first time for shares of a financial holding to be listed on a Japanese stock exchange.
On their last day of trading Thursday, IBJ, DKB and Fuji Bank were the top three volume leaders on the first section.
The TSE took into account Thursday's closing prices of the three banks to set the standard price of Mizuho Holdings shares at 817,000 yen.
As the merger ratio is equal among the three banks, an investor who holds 1,000 shares in any of the three will receive one share in Mizuho Holdings, with a face value of 50,000 yen.
In a related development, DKB and Fuji Bank delisted their common shares from the London Stock Exchange on Thursday.