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A missile was fired into the sky on Friday on Turkey’s Black Sea coast where the military was expected to test its Russian-made S-400 defense systems, according to local video obtained by Reuters, drawing a strong warning from the United States.

The video, taken in the coastal city of Sinop, showed a narrow column of smoke headed high into the blue sky. In recent days Turkey had issued notices restricting airspace and waters off the coastal area to allow firing tests.

A U.S. official, speaking on the condition of anonymity, said Turkey had tested the S-400 system on Friday but did not provide details.

Tests of the S-400s, if verified, could stoke tensions between Turkey and the United States, which sharply opposed Ankara’s purchase of the weapons from Moscow on grounds they compromise shared NATO defense systems.

State Department spokeswoman Morgan Ortagus said the United States has expressed to the most senior levels of the Turkish government that the acquisition of Russian military systems such as the S-400 is unacceptable, adding that Washington has been clear on its expectation that the system should not be operationalized.

“If confirmed, we would condemn in the strongest terms the S-400 test missile launch as incompatible with Turkey’s responsibilities as a NATO  Ally and strategic partner of the United States,” Ortagus added.

The Pentagon, separately, said that the S-400 should not be activated.

“Turkey has already been suspended from the F-35 program and the S-400 continues to be a barrier to progress elsewhere in the bilateral relationship,” a Pentagon spokesman said.

Turkey’s Defense Ministry said it would neither deny nor confirm missile tests.

Washington reacted last year by suspending Turkey from its F-35 jet program and has threatened sanctions.

Defense analyst Turan Oguz said a preliminary assessment of the color, intensity, angle and route of the smoke in the video coincided with S-400 missiles. The angle of the column suggested the target “must not be too high,” he added.

Last year the military conducted radar tests of the surface-to-air defenses, which is among the world’s most advanced and can spot and track incoming aircraft at medium and long ranges.

Turkey signed the S-400 deal with Russia in 2017. Deliveries of the first four missile batteries, worth $2.5 billion, began in July last year.

U.S. Sen. Jim Risch, chairman of the Senate Foreign Relations Committee, called the test “unacceptable behavior” from a NATO ally.

In a statement, Risch said the move damages the alliance and posed a direct threat to the F-35 and other U.S. and NATO allies’ systems.

“U.S. law requires sanctions against countries that continue to deepen their defense relationship with Russia, and the administration should send a strong signal that Turkey must divest its S-400s,” he said.

U.S. Sen. Bob Menendez, the ranking Democrat on the Senate Foreign Relations Committee, said President Donald Trump’s affinity for Turkish President Recep Tayyip Erdogan posed a serious threat to the United States’ national security.

“Turkey must be sanctioned immediately for its purchase and use of this system,” Menendez said in a statement.

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