GRAVOBO, UKRAINE/WASHINGTON – U.S. President Barack Obama said the downing of a Malaysian jetliner in a Ukrainian region controlled by Russian-backed separatists should be a “wake-up call for Europe and the world” in a crisis that appears to be at a turning point and warned Russia of possible tightening of sanctions.

While stopping short of blaming Russia for Thursday’s crash of Malaysia Airlines Flight MH17, in which all 298 people died, Obama accused Moscow of failing to stop the violence that made it possible to shoot down the plane.

The United States has said the jetliner was hit by a surface-to-air missile fired from rebel territory.

A senior U.S. official said there is increasing confidence that the missile was fired by separatists and that there is no reason to doubt the validity of a widely circulated audiotape in which voices identified as separatists discussed the downing of the plane.

“This certainly will be a wake-up call for Europe and the world that there are consequences to an escalating conflict in eastern Ukraine; that it is not going to be localized, it is not going to be contained,” Obama told reporters Friday.

Obama later spoke by phone with German Chancellor Angela Merkel, British Prime Minister David Cameron and Australian Prime Minister Tony Abbott. The White House said they discussed Ukraine and the downed jet and the need for an unimpeded international investigation into what happened.

Malaysian Transport Minister Liow Tiong Lai said Saturday he will fly to Kiev, the Ukrainian capital, to ensure an investigating team gets safe access to the site.

Defense Minister and former Transport Minister Hishammuddin Hussein said a main priority is to ensure debris is not tampered with.

“We want to get to the bottom of this,” he added, saying that Malaysia has been in contact with officials in Russia, Ukraine, the United States, Britain and China.

“We do not have a position until the facts have been verified, whether the plane was really brought down, how it was brought down, who brought it down.”

International observers said gunmen stopped them examining the site properly when they got there on Friday. More than half of the victims were Dutch in what has become a pivotal incident in deteriorating relations between Russia and the West.

Obama ruled out military intervention, but said he is prepared to tighten sanctions.

Russia, which Obama said is letting the rebels bring in weapons, has expressed anger at implications it is to blame, saying people should not prejudge the outcome of an inquiry.

There were no survivors from Flight MH17 from Amsterdam to Kuala Lumpur, a Boeing 777. The United Nations said 80 of the 298 aboard were children. The deadliest attack on a commercial airliner, it scattered bodies over a large area of rebel-held territory near the border with Russia.

The loss is the second devastating blow for Malaysia Airlines and the country this year, following the disappearance of Flight MH370 in March with 239 passengers and crew on board on its way from Kuala Lumpur to Beijing.

Makeshift white flags marked where bodies lay in corn fields and among the debris. Others, stripped bare by the force of the crash, had been covered by polythene sheeting weighed down by stones, one marked with a flower in remembrance.

One pensioner told how a the body of a woman smashed though the roof of her house. “There was a howling noise and everything started to rattle. Then objects started falling out of the sky,” said Irina Tipunova, 65. “And then I heard a roar and she landed in the kitchen.”

As U.S. investigators prepared to head to Ukraine to assist in the investigation, staff from the Organization for Security and Co-operation in Europe (OSCE) visited the site but complained that they did not get the full access they wanted.

“We encountered armed personnel who acted in a very impolite and unprofessional manner. Some of them even looked slightly intoxicated,” an OSCE spokesman said.

The scale of the disaster could prove a turning point for international pressure to resolve the crisis in Ukraine, which has killed hundreds since pro-Western protests toppled Moscow-backed President Viktor Yanukovych in Kiev in February and Russia annexed the Crimea Peninsula a month later.

“This outrageous event underscores that it is time for peace and security to be restored in Ukraine,” Obama said, adding that Russia has failed to use its influence to curb rebel violence.

While the West has imposed sanctions on Russia over Ukraine, the United States has been more aggressive than the European Union. Analysts say the response of Germany and other EU powers to the incident — possibly imposing more sanctions — could be crucial in deciding the next phase of the standoff with Moscow.

The U.N. Security Council called for a “full, thorough and independent international investigation” into the downing of the plane and “appropriate accountability” for those responsible.

Kiev and Moscow immediately blamed each other for the disaster, triggering a new phase in their propaganda war.

The plane crashed about 40 km (25 miles) from the border with Russia near the regional capital of Donetsk, an area that is a stronghold of rebels who have been fighting Ukrainian government forces and have brought down military aircraft.

Leaders of the rebels’ self-proclaimed “Donetsk People’s Republic” denied any involvement and said a Ukrainian Air Force jet had shot down the plane.

Russia’s Defense Ministry later laid the blame with Ukrainian ground forces, saying it had picked up radar activity from a Ukrainian missile system south of Donetsk when the airliner was brought down, Russian media reported.

The Ukrainian security council said no missiles had been fired from its armories. Officials also accused separatists of moving unused missiles into Russia after the incident.

The Ukrainian government released recordings it said were of Russian intelligence officers discussing the shooting down of a civilian airliner by rebels who may have mistaken it for a Ukrainian military plane.

The OSCE monitors said they could not find anyone to talk to about the plane’s two black boxes- voice and data recorders — and villagers were seen removing pieces of wreckage.4/50/2 medium OVERLINE

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1. chris

2.The Malaysia Airlines Boeing 777 with the tail number 9M-MRD — the same aircraft that was shot down near the Ukraine-Russia border Thursday — takes off from Los Angeles International Airport in November 2012. AP